This is not an x-ray but is a beautifully prepared skeleton of a juvenile pumpkinseed fish specimen (yeah that’s its actual name lmao ). Clearing and Staining is a preparative method used by scientists and biologists to create and study intricate skeletons by carefully rendering the flesh invisible, turning bone red, and the cartilage blue.
Scientists who utilize this method do so to study the anatomical relation of skeletons and how they interact with soft tissue (shape and morphology). By looking at form and understanding formation of animals, we can understand function and how biological systems work harmoniously with each other. 3D CT scans are awesome but they don’t visualize soft tissue. MRI’s are too low resolution, and histology (slicing things and reconstructing them) doesn’t give you an inherent 3d sense. Big advantage of clearing and staining is it gives you beautiful 3D reconstruction of atoms in the animal and it shows mesmerizing detail of bone and cartilage by being non-invasive.
You may be thinking that this process is expensive but it is cheaper than any other methods mentioned above and you can do it yourself at home if you are into that sort of stuff and thangs. Alright the first thing you do is you solidify all the tissues (in 10-15% Formalin) and then you stain the cartilage in blue. Then you stain the bone in red. At this point the fish looks just like a fish. Then half magic happens when we put the fish in trypsin which eats up basically almost everything in the fish except collagen (collagen is the same stuff you find in your skin and it helps hold the fish together). Then a little bit of bleach removes dark colors and you are left with a white fish Now full magic happens and everything comes together when we put the fish in glycerin (clear viscous fluid often used in foods as sweetener and preservative. It is the fluid you see in the bottle in 2nd picture). This causes the entire fish to turn transparent because collagen has the same refractive index as glycerin.
Focal Length: 150mm
Shutter Speed: 1/160 sec
#nofilter #fish #beauty #nature #science #lab #nikon #macro #photography #biology